Table 2 Estimation of PLG
Strain | M. bovis(gm) | M. smeg(gm) | MSFP (gm) | MSP1 (gm) | MSP2 (gm) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | LN | HN | LN | HN | LN | HN | LN | HN | LN | HN |
Dry cell weight | 2.78±0.3 | 2.85±0.2 | 3.04±0.4 | 3.3±0.19 | 3.876±0.16 | 3.34±0.18 | 2.98±0.24 | 3.008±0.11 | 3.43±0.14 | 3.07±0.25 |
Cell wall weight after sonication | 1.08±0.2 | 1.34±0.1 | 1.24±0.15 | 1.43±0.23 | 1.87±0.11 | 1.56±0.12 | 1.32±0.32 | 1.47±0.07 | 1.36±0.11 | 1.57±0.11 |
Insoluble cell wall after SDS extraction and acetone wash | 0.870±0.1 | 0.680±0.08 | 0.768±0.08 | 0.567±0.13 | 1.02±0.2 | 0.98±0.14 | 0.69±0.09 | 0.75±0.08 | 0.62±0.07 | 0.73±0.12 |
Poly-L-glutamine pelleted after sucrose gradient centrifugation | 0.070±0.03 | No Pellet | No Pellet | No Pellet | 0.087±0.017 | No Pellet | 0.078±0.011 | No Pellet | 0.056±0.02 | No Pellet |
Poly-L-glutamine purified after percoll run | 0.069±0.02 | No PLG | No PLG | No PLG | 0.075±0.012 | No PLG | 0.056±0.02 | No PLG | 0.034±0.01 | No PLG |