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Table 1 Comparison of m/z values of N-terminal AspN-digested/tryptic peptides of LprF, LpqH, LpqL and LppX found in BCG parental and Δ lnt mutant strain

From: Lipoproteins of slow-growing Mycobacteria carry three fatty acids and are N-acylated by Apolipoprotein N-Acyltransferase BCG_2070c

  Peptide Calculated m/z Parental strain m/z Δlnt m/z
LprF CGK…ILQ 2496.24 - -
CGK…ILQ 3047.11 - 3046.70
 + Diacylglycerol (C16/C16) (+550.87)   (+550.46)
CGK…ILQ 3073.15 - 3072.71
 + Diacylglycerol (C16/C18) (+576.91)   (+576.47)
CGK…ILQ 3089.20 - 3088.74
 + Diacylglycerol (C16/C19) (+592.96)   (+592.50)
CGK…ILQ 3251.44 - 3251.65
 + Diacylglycerol (C16/C19) (+755.20)   (+755.41)
 + Hexose    
CGK…ILQ 3327.60 3326.83 -
 + Diacylglycerol (C16/C19) (+831.36) (+830.59)  
 + N-acyl (C16)    
CGK…ILQ 3531.93 3530.56 -
 + Diacylglycerol (C16/C19) (+1035.69) (+1034.32)  
 + N-acyl (C19)    
   + Hexose    
LpqH CSSNK 538.23 - -
CSSNK 1089.10 - 1088.60
 + Diacylglycerol (C16/C16) (+550.87)   (+550.37)
CSSNK 1115.14 - 1114.68
 + Diacylglycerol (C16/C18) (+576.91)   (+576.45)
CSSNK 1131.19 1130.79 1130.71
 + Diacylglycerol (C16/C19) (+592.96) (+592.56) (+592.48)
CSSNK 1369.59 1369.04 -
 + Diacylglycerol (C16/C19) (+831.36) (+830.81)  
   + N-acyl (C16)    
LpqL CIR 391.21 - -
CIR 984.17 984.50 983.77
 + Diacylglycerol (C16/C19) (+592.96) (+593.29) (+592.56)
CIR 1222.57 1221.98 -
 + Diacylglycerol (C16/C19) (+831.36) (+830.77)  
   +N-acyl (C16)    
LppX CSS…EIR 2964.46 - -
CSS…EIR 3515.33 3514.94 3514.94
 + Diacylglycerol (C16/C16) (+550.87) (+550.48) (+550.48)
CSS…EIR 3557.42 - 3556.96
 + Diacylglycerol (C16/C19) (+592.96)   (+592.50)
CSS…EIR 3719.66 - 3719.00
 + Diacylglycerol (C16/C19) (+755.20)   (+754.54)
 +Hexose    
CSS…EIR 3795.82 3795.21 -
 + Diacylglycerol (C16/C19) (+831.36) (+830.75)  
 + N-acyl (C16)    
CSS…EIR 3881.90 - 3881.06
 + Diacylglycerol (C16/C19) (+917.44)   (+916.60)
 + 2 Hexoses    
CSS…EIR 3958.06 3957.28 -
 + Diacylglycerol (C16/C19) (+993.60) (+992.82)  
 + N-acyl (C16)    
 + Hexose    
CSS…EIR 4120.30 4119.45 -
 + Diacylglycerol (C16/C19) (+1155.84) (+1154.99)  
 + N-acyl (C16)    
   + 2 Hexoses    
  1. Peptides correspond to the N-terminal AspN-digested/tryptic peptides of LprF, LpqH, LpqL and LppX upon cleavage of the signal peptide by LspA. Mass differences to the corresponding unmodified peptide (bold number) due to modifications are given in parentheses. Observed modifications are: diacylglycerol with C16 fatty acid and C16 fatty acid (+550.87 Da). Diacylglycerol with C16 fatty acid and tuberculostearic acid (C19:0) (+592.96 Da), plus one hexose (+162.24 Da, Σ = 755.20 Da) or two hexoses (+324.48 Da, Σ = 917.44). Diacylglycerol with C16 fatty acid and C19:0 fatty acid (+592.96 Da) plus N-acyl with C16 fatty acid (+238.40 Da, Σ = 831.36), N-acyl with C16 fatty acid plus one hexose (+162.24 Da, Σ = 993.6 Da) or two hexoses (+324.48 Da, Σ = 1155.84 Da). Or diacylglycerol with C16 fatty acid and C19:0 fatty acid (+592.96 Da) plus N-acyl with C19:0 fatty acid and hexose (+280.49 Da +162.24 Σ = 1035.69).