Table 1 Comparison of m/z values of N-terminal AspN-digested/tryptic peptides of LprF, LpqH, LpqL and LppX found in BCG parental and Δ lnt mutant strain
|  | Peptide | Calculated m/z | Parental strain m/z | Δlnt m/z |
|---|---|---|---|---|
LprF | CGK…ILQ | 2496.24 | - | - |
CGK…ILQ | 3047.11 | - | 3046.70 | |
 + Diacylglycerol (C16/C16) | (+550.87) |  | (+550.46) | |
CGK…ILQ | 3073.15 | - | 3072.71 | |
 + Diacylglycerol (C16/C18) | (+576.91) |  | (+576.47) | |
CGK…ILQ | 3089.20 | - | 3088.74 | |
 + Diacylglycerol (C16/C19) | (+592.96) |  | (+592.50) | |
CGK…ILQ | 3251.44 | - | 3251.65 | |
 + Diacylglycerol (C16/C19) | (+755.20) |  | (+755.41) | |
 + Hexose |  |  |  | |
CGK…ILQ | 3327.60 | 3326.83 | - | |
 + Diacylglycerol (C16/C19) | (+831.36) | (+830.59) |  | |
 + N-acyl (C16) |  |  |  | |
CGK…ILQ | 3531.93 | 3530.56 | - | |
 + Diacylglycerol (C16/C19) | (+1035.69) | (+1034.32) |  | |
 + N-acyl (C19) |  |  |  | |
|  |  + Hexose |  |  |  |
LpqH | CSSNK | 538.23 | - | - |
CSSNK | 1089.10 | - | 1088.60 | |
 + Diacylglycerol (C16/C16) | (+550.87) |  | (+550.37) | |
CSSNK | 1115.14 | - | 1114.68 | |
 + Diacylglycerol (C16/C18) | (+576.91) |  | (+576.45) | |
CSSNK | 1131.19 | 1130.79 | 1130.71 | |
 + Diacylglycerol (C16/C19) | (+592.96) | (+592.56) | (+592.48) | |
CSSNK | 1369.59 | 1369.04 | - | |
 + Diacylglycerol (C16/C19) | (+831.36) | (+830.81) |  | |
|  |  + N-acyl (C16) |  |  |  |
LpqL | CIR | 391.21 | - | - |
CIR | 984.17 | 984.50 | 983.77 | |
 + Diacylglycerol (C16/C19) | (+592.96) | (+593.29) | (+592.56) | |
CIR | 1222.57 | 1221.98 | - | |
 + Diacylglycerol (C16/C19) | (+831.36) | (+830.77) |  | |
|  |  +N-acyl (C16) |  |  |  |
LppX | CSS…EIR | 2964.46 | - | - |
CSS…EIR | 3515.33 | 3514.94 | 3514.94 | |
 + Diacylglycerol (C16/C16) | (+550.87) | (+550.48) | (+550.48) | |
CSS…EIR | 3557.42 | - | 3556.96 | |
 + Diacylglycerol (C16/C19) | (+592.96) |  | (+592.50) | |
CSS…EIR | 3719.66 | - | 3719.00 | |
 + Diacylglycerol (C16/C19) | (+755.20) |  | (+754.54) | |
 +Hexose |  |  |  | |
CSS…EIR | 3795.82 | 3795.21 | - | |
 + Diacylglycerol (C16/C19) | (+831.36) | (+830.75) |  | |
 + N-acyl (C16) |  |  |  | |
CSS…EIR | 3881.90 | - | 3881.06 | |
 + Diacylglycerol (C16/C19) | (+917.44) |  | (+916.60) | |
 + 2 Hexoses |  |  |  | |
CSS…EIR | 3958.06 | 3957.28 | - | |
 + Diacylglycerol (C16/C19) | (+993.60) | (+992.82) |  | |
 + N-acyl (C16) |  |  |  | |
 + Hexose |  |  |  | |
CSS…EIR | 4120.30 | 4119.45 | - | |
 + Diacylglycerol (C16/C19) | (+1155.84) | (+1154.99) |  | |
 + N-acyl (C16) |  |  |  | |
|  |  + 2 Hexoses |  |  |  |