Figure 2From: Development of dual-function ELISA for effective antigen and antibody detection against H7 avian influenza virusSpecificity of H7 antigen detection in the dual ELISA. The specificity of H7 antigen detection in the dual-ELISA was examined with 100 ul of PBS containing the H7 strains adjusted to an HA titer of 8 or non-H7 viruses with HA titers of ≥16. Values represent the means of absorbances of duplicate wells from two independent tests. OD 490: optical density at 490 nm; dotted line: cut-off values; Blank AF: allantoic fluid without virus.Back to article page