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Table 2 MIC data for lacticin 3147, and its individual peptides Ltnα and Ltnβ, polymyxin B and polymyxin E alone and in combination

From: The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria

E.coli0157:H-
MIC (μg/ml)
Lacticin 3147 Polymyxin B Polymyxin E Lacticin 3147/ FIC Lacticin 3147/ FIC
Polymyxin B Polymyxin E
231 (37.5 μM) 0.0586 0.0781 28.875/0.0073 0.250a 28.875/ 0.0049 0.188a
(α :124.74, Β: 106.26)    28.875 / 0.0147* 0.376*a 14.4375 / 0.0195* 0.312*a
Ltnα Polymyxin B Polymyxin E Ltnα/ FIC Ltnα/ FIC
Polymyxin B Polymyxin E
187.11 (56.25 μM) 0.0586 0.0781 93.555 / 0.0073 0.625b 46.7775/ 0.0195 0.500a
(1.5 X Ltnα)    (6.0 X Ltnα in combin.)   (6.0 X Ltnα in combin.)  
Ltnβ Polymyxin B Polymyxin E Ltnβ/ FIC Ltnβ/ FIC
Polymyxin B Polymyxin E
495.88 (175 μM) 0.0586 0.0781 61.9850 / 0.0147 0.376a 30.9925 / 0.0195 0.313a
(4.7 X Ltnβ)    (4.7 X Ltnβ in combin.)   (4.7 X Ltnβ in combin.)  
  1. FIC figures have been calculated as a result of triplicate experiments and indicate asynergy and bpartial synergy effects.*Alternative MIC and FIC data that allow for fixed levels of polymyxin across antimicrobial combinations, thus allowing for the calculation of the involvement of Ltnα and Ltnβ in synergy with polymyxin.