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BMC Microbiology

Table 2 MIC data for lacticin 3147, and its individual peptides Ltnα and Ltnβ, polymyxin B and polymyxin E alone and in combination

From: The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria

*E.coli*0157:H-
MIC (μg/ml)
Lacticin 3147	Polymyxin B	Polymyxin E	Lacticin 3147/	FIC	Lacticin 3147/	FIC
Lacticin 3147	Polymyxin B	Polymyxin E	Polymyxin B	FIC	Polymyxin E	FIC
231 (37.5 μM)	0.0586	0.0781	28.875/0.0073	0.250^a	28.875/ 0.0049	0.188^a
(α :124.74, Β: 106.26)			28.875 / 0.0147*	0.376*^a	14.4375 / 0.0195*	0.312*^a
Ltnα	Polymyxin B	Polymyxin E	Ltnα/	FIC	Ltnα/	FIC
Ltnα	Polymyxin B	Polymyxin E	Polymyxin B	FIC	Polymyxin E	FIC
187.11 (56.25 μM)	0.0586	0.0781	93.555 / 0.0073	0.625^b	46.7775/ 0.0195	0.500^a
(1.5 X Ltnα)			(6.0 X Ltnα in combin.)		(6.0 X Ltnα in combin.)
Ltnβ	Polymyxin B	Polymyxin E	Ltnβ/	FIC	Ltnβ/	FIC
Ltnβ	Polymyxin B	Polymyxin E	Polymyxin B	FIC	Polymyxin E	FIC
495.88 (175 μM)	0.0586	0.0781	61.9850 / 0.0147	0.376^a	30.9925 / 0.0195	0.313^a
(4.7 X Ltnβ)			(4.7 X Ltnβ in combin.)		(4.7 X Ltnβ in combin.)

FIC figures have been calculated as a result of triplicate experiments and indicate ^asynergy and ^bpartial synergy effects.*Alternative MIC and FIC data that allow for fixed levels of polymyxin across antimicrobial combinations, thus allowing for the calculation of the involvement of Ltnα and Ltnβ in synergy with polymyxin.

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