Figure 5
From: Identification of conserved motifs in the Westnile virus envelope essential for particle secretion
Depletion of endogenous Tsg101 or Alix using specific siRNA does not inhibit WNV release. 293T cells were transfected with control, Alix or Tsg101 siRNA. 24 h post transfection cells were transfected again with respective siRNAs along with (A) WT HIV-1 pNL4-3 DNA (B) WT EIAV Gag DNA or (C) WNV-CPrME plus the Ren/Rep plasmids. Virus release was determined after radiolabeling and immunoprecipitation for HIV and WNV, via western blotting for EIAV and also by the rapid ren-luc based assay for WNV. Data represent mean ± SD from 3 independent experiments (A&C). For the ren-luc based WNV assay one representative of 3 independent experiments is shown.