Table 1 Genotype of bacterial strains, plasmids and ICE R391 mutants used

AB1157

F^-, thr-1, araC14, leuB6, ∆(gpt-proA)62, lacY1, tsx-33, qsr’-0, glnV44, galK2, λ-, Rac-0, hisG4, rfbC1, mgl-51, rpoS396, rpsL31 (Str^R), kdgK51, xylA5, mtl-1, argE3, thi-1

E. coli genetic stock centre (CGSC), Yale University, New Haven, Connecticut, USA

TOP10

F^-, mcrA0, ∆(mrr-hsdRMS-mcrBC), φ80dlacZ58(M15), ∆lacX74, recA1, araD139, ∆(araA-leu)7697, galU^-, galK0, rpsL^- (Str^R), endA1, nupG^-

Bio-Sciences, Dun Laoghaire, Dublin, Ireland

P125109

S. Enteritidis PT4 wild type (NCTC 13349), Nal^R

National Collection of Type Cultures (NCTC), Salisbury, UK

Plasmid

Genotype

Source

pBAD33-orf43

Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43

Armshaw and Pembroke, 2013 [8]

pBAD33-orf43[SM12]

Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43 containing mutation converting two leucines to prolines at a.a. position 47 and 48.

This study

pBAD33-orf43[SM56]

Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43 containing mutation converting glutamine at position 115 to asparagine.

This study

pKOBEG

T_s, P_BAD-gam-bet-exo cat (Cm^R)

Dr. P. Latour-Lambert, Institut Pasteur, 25 rue du Dr Roux, Paris, France

pUC18

Am^R template for deletion mutant construction

Sigma-Aldrich, Arklow, Wicklow, Ireland

pcDNA3.1⁽⁺⁾

Ze^R template for deletion mutant construction

Invitrogen, Bio-Sciences, Dun Laoghaire, Dublin, Ireland

ICE

Genotype

Source

R391

Km^R, Hg^R

Dr R.W. Hedges, Royal Postgraduate Medical School, London, UK

R391 Mutant

Genotype

Source

AB1157 R391 ∆14 (∆orf43)

ICE R391 orf43 deletion strain, Am^R, UV^-, tra^-

Armshaw and Pembroke, 2013 [8]

AB1157 R391 ∆26 (∆orfs90/91)

ICE R391 orfs90/91 deletion strain, Am^R, UV^-, tra^-

Armshaw and Pembroke, 2013 [8]

AB1157 R391 ∆11 (∆orfs40/41)

ICE R391 orfs40/41 deletion strain, Am^R, tra^-

Armshaw and Pembroke, 2013 [8]

AB1157 R391 ∆25_Am^R∆14_Ze^R

ICE R391 orf90 – orf94 and orf43 deletion strain, Am^R, Ze^R, UV^-, tra^-

This study

AB1157 R391 KOA

ICE R391 orf32 - orf42 (29575 bp – 41491 bp) deletion strain, Am^R, tra^-

This study

AB1157 R391 KOB

ICE R391 orf32 - orf42 (29575 bp – 41527 bp) deletion strain, Am^R, UV^-, tra^-

This study

AB1157 R391 KOC

ICE R391 orf32 - orf42 (29575 bp – 41491 bp) and orfs90/91 deletion strain, Am^R, Ze^R, UV^-, tra^-

This study