Table 2 Primers used in the study

†Lactobacillus –Pediococcus-Leuconostoc-Weissella (Lactobacillus group) (341 bp)

Lac1: AGC AGT AGG GAA TCT TCC A

62

[39, 40]

Lab667r: CAC CGC TAC ACA TGG AG

†Enterococcus spp.(144 bp)

Ent-F: CCC TTA TTG TTA GTT GCC ATC ATT

60

[41]

Ent-R: ACT CGT TGT ACT TCC CAT TGT

†Enterobacteriaceae (195 bp)

Enterobac-F: CAT TGA CGT TAC CCG CAG AAG AAG C

63

[42]

Enterobac-R: CTC TAC GAG ACT CAA GCT TGC

†Staphylococcus spp. (370 bp)

TStaG422: GGC CGT GTT GAA CGT GGT CAA ATC

55

[43]

TStaG765: TIA CCA TTT CAG TAC CTT CTG GTA A

†Bacillus spp. (995 bp)

BacF: GGGAAACCGGGGCTAATACCGGAT

55

[44]

BacR: GTC ACC TTA GAG TGC CC

†E. coli (544 bp)

ECP79F: GAA GCT TGC TTC TTT GCT

54

[45]

ECP620R: GAG CCC GGG GAT TTC ACA T

†SLT-I (614 bp)

VT1 (SLTI-F): ACA CTG GAT GAT CTC AGT GG

55

[44]

VT2 (SLTI-R): CTG AAT CCC CCT CCA TTA TG

†SLT-II (779 bp)

VT3 (SLTII-F): CCA TGA CAA CGG ACA GCA GTT

55

VT4 (SLTII-R): CCT GTC AAC TGA GCA CTT T

16S rDNA Sequencing

616V: AGA GTT TGA TYM TGG CTC

52

[46]

(~1500 bp)

630R: AAG GAG GTG GAT CCA RCC

CAKAAAGGAGGTGGATCC

Random Primer for RAPD

DAF4: CGG CAG CGC C

35

[47]

M13V: GTT TTC CCA GTC ACG ACG TTG

35

[48]

Universal Primers

HDA1: ACT CCT ACG GGA GGC AGC AG

52

[49]

HDA2: GTA TTA CCG CGG CTG CTG GCA

HDA1 + GC: CGC CCG GGG CGC GCC CCG GGC GGG GCG GGG GGC ACG GGG GGA CTC CTA CGG GAG GCA GCA G

TA Cloning

M13Forward (−20): GTA AAA CGA CGG CCA G

55

[50]

M13Reverse: CAG GAA ACA GCT ATG AC

†Pediocin Structural Gene pedA (100 bp)

pedA2RTF: GGC CAA TAT CAT TGG TGG TA

60

[25]

pedA2RTR: ATT GAT TAT GCA AGT GGT AGC C

TqM-pedA: FAM-ACT TGT GGC AAA CAT TCC TGC

TCT GTT GA-TAMRA

†Total Bacteria (727 bp)

TotalBac-F785: GGA TTA GAT ACC CTG GTA GTC

52

[51–53]

TotalBac-R1512r: TAC CTT GTT ACG ACT T

TaqMan 1400r Probe: 6-FAM-TGA CGG GCG GTG TGT ACA AGG C-TAMRA