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Table 1 Selected proteins differentially expressed during X. a. pv. citri biofilm formation

From: Insights into xanthomonas axonopodis pv. citri biofilm through proteomics

Spot no. Protein name MOWSE score Accession no. Species Gene ID in Xaca Predicted MW/pI Observed MW/pI Peptide match/
coverage
Fold changeb
01 Metabolism
01.02 Nitrogen, sulfur and selenium metabolism
01.02.02 Nitrogen metabolism
60 NAD(PH) nitroreductase 111 Y587_XANC5 X. c. pv. vesicatoria XAC0554 21.0/5.83 20.0/4.6 7/31% −5.6
01.05 C-compounds and carbohydrate metabolism
220 UDP-glucose dehydrogenase 125 Q8PGN5_XANAC X. a. pv. citri XAC3581 43.1/6.18 68.0/6.7 13/25% +2.6
01.06 Lipid, fatty acid and isoprenoid metabolism
01.06.02 Membrane lipid metabolism
609 Outer membrane protein (FadL) 1070 Q8PRE4_XANAC X. a. pv. citri XAC0019 47.3/5.18 54.0/6.0 54/40% +2.6
01.20 Secondary metabolism
533 Coproporphyinogen-III oxidase, aerobic 191 HEM6_XANAC X. a. pv. citri XAC4109 34.6/5.81 48.0/5.4 11/30% −1.5
434 Short chain dehydrogenase 141 Q8PME5_XANAC X. a. pv. citri XAC1484 26.0/5.97 29.0/4.5 14/34% −5.1
02 Energy
02.04 Glyoxylate cycle
331 KDPG and KHG aldolase 163 Q8PKU5_XANAC X. a. pv. citri XAC2067 22.9/5.24 23.0/4.8 7/31% −2.0
02.10 Tricarboxylic-acid pathway
98 Malate dehydrogenase 905 MDH_XANAC X. a. pv. citri XAC1006 34.9/5.37 48.0/4.3 46/51% +1.5
121 Dihydrolipoamide S-succinyltransferase 136 Q3BVA5_XANC5 X. c. pv. vesicatoria XAC1534 42.4/5.87 69.0/6.5 9/10% +1.8
235 Citrate synthase 218 Q3BPS8_XANC5 X. c. pv. vesicatoria XAC3388 47.9/5.97 68.0/6.6 8/20% +2.6
591 Succinate dehydrogenase flavoprotein subunit 206 Q3BTD_XANC5 X. c. pv. vesicatoria XAC2077 65.8/5.89 55.0/4.4 18/22% −7.4
02.45 Energy conversion and regeneration
02.45.15 Energy generation
76 ATP synthase beta chain 72 Q2P7Q4_XANOM X. o. pv. oryzae XAC3649 51.0/5.18 32.0/6.1 3/8% −10.7
442 Phosphoglycerate kinase 688 AAM38190 X. a. pv. citri XAC3347 40.9/4.91 45.0/6.0 47/43% −1.9
422 NADH-ubiquinone oxidoreductase 40 Q3BRN4_XANC5 X. c. pv. vesicatoria XAC2699 48.8/6.32 33.0/4.4 8/18% −3.9
11 Transcription
11.04 RNA processing
153 Polynucleotide phosphorylase 137 PNP_XANAC X. a. pv. citri XAC2683 75.5/5.47 28.0/5.9 6/3% −1.5
12 Protein synthesis
12.01 Ribosome biogenesis
79 50S ribosomal protein L4 133 AAM35856 X. a. pv. citri XAC0973 21.8/9.68 14.0/5.9 4/15% +5.1
12.04 Translation
26 Elongation factor Tu 294 Q3BWY6_XANC5 X. c. pv. vesicatoria XAC0957 43.3/5.45 67.0/6.2 25/24% +2.2
173 Elongation factor Tu 329 Q3BWY6_XANC5 X. c. pv. vesicatoria XAC0957 43.3/5.45 48.0/5.9 20/42% +4.4
14 Protein fate (folding, modification and destination)
14.01 Protein folding and stabilization
416 Chaperone protein DnaK 98 DNAK_XANOM X. o. pv. oryzae XAC1522 68.9/5.02 66.0/6.3 10/12% +2.9
20 Cellular transport, transport facilities and transport routes
20.03 Transport facilities
151 Regulator of pathogenicity factors 104 Q8PJM6_XANAC X. a. pv. citri XAC2504 41.3/5.98 41.0/4.3 8/21% +3.2
429 Regulator of pathogenecity factors 729 Q8PJM6_XANAC X. a. pv. citri XAC2504 41.3/5.98 47.0/4.5 55/61% +2.7
486 Regulator of pathogenecity factors 231 Q8PJM6_XANAC X. a. pv. citri XAC2504 41.3/5.98 48.0/5.2 16/30% +2.2
526 *Regulator of pathogenecity factors 183 Q3BS50_XANC5 X. c. pv. vesicatoria XAC2504 46.4/7.10 48.0/5.3 16/21% +1.8
555 *Regulator of pathogenecity factors 148 Q3BS50_XANC5 X. c. pv. vesicatoria XAC2504 46.4/7.10 42.0/4.9 11/12% +2.8
30 Cellular communication/Signal transduction mechanism
103 OmpA-related protein 371 Q8PER6_XANAC X. a. pv. citri XAC4274 110.1/5.29 75.0/5.9 28/16% +2.9
1 TonB-dependent receptor 1406 Q8PI48_XANAC X. a. pv. citri XAC3050 105.8/4.76 42.0/4.1 89/34% +2.9
2 TonB-dependent receptor 1441 Q8PI48_XANAC X. a. pv. citri XAC3050 105.8/4.76 58.0/6.7 85/35% +2.9
74 TonB-dependent receptor 597 Q8PI48_XANAC X. a. pv. citri XAC3050 105.8/4.76 20.0/4.7 27/15% +3.4
219 TonB-dependent receptor 356 Q8PI48_XANAC X. a. pv. citri XAC3050 105.8/4.76 68.0/6.4 23/23% +2.2
466 TonB-dependent receptor-precursor 113 Q8PI27_XANAC X. a. pv. citri XAC3071 97.3/5.14 54.0/6.8 7/4% +3.6
55 *TonB-dependent receptor 166 Q2HPF0_9XANT X. a. pv. glycines XAC3489 88.9/4.93 58.0/6.4 8/9% +2.8
168 TonB-dependent receptor 636 Q8PGX3_XANAC X. a. pv. citri XAC3489 89.0/5.00 55.0/6.0 38/29% +4.9
38 *TonB-dependent receptor 594 Q8PHT1_XANAC X. a. pv. citri XAC3168 87.3/5.20 48.0/6.0 44/21% −1.8
15 TonB-dependent receptor 229 Q8PH16_XANAC X. a. pv. citri XAC3444 103.2/4.79 66.0/6.4 20/14% −3.5
30.01.05.01 Protein kinase
49 Adenylate kinase 93 Q3BPM9_XANC5 X. c. pv. vesicatoria XAC3437 19.9/5.33 18.0/5.9 8/24% −2.4
420 Histidine kinase- 2 component sensor system 40 Q3BTZ4_XANC5 X. c. pv. vesicatoria XAC1991 45.9/5.33 48.0/5.5 10/13% −2.2
34 Interaction with the environment
86 YapH protein 51 Q8PKM0_XANAC X. a. pv. citri XAC2151 306.9/4.15 68.0/5.5 1/0% +4.1
42 Biogenesis of cellular components
42.27 Extracellular/secretion protein
432 OmpW family outer memb. prot. precursor 151 Q3BP00_XANC5 X. c. pv. vesicatoria XAC3664 23.8/4.97 17.0/6.1 5/13% +2.2
  1. a Gene accession number in X. axonopodis pv. citri genome of the identified protein.
  2. b Fold change in biofilm compared to planktonic cultures.
  3. * Protein spots 55 and 38 were previously identified as “outer membrane active sucrose transporter” and “ferric enterobactin receptor” are now classified as TonB-dependent receptor, while protein spots 526 and 555 were previously identified as “carbohydrate selective porin” and is now classified as Regulator of pathogenecity factors.