| Km(μM) | kcat(s-1) | kcat/Km(s-1M-1)×103 |
---|
Human TK1 | 5.9 ± 1.7 | 0.043 ± 0.003 | 7.3 ± 1.8 |
Human TK2 | 8.8 ± 3.8 | 0.026 ± 0.003 | 3.0 ± 0.8 |
Ureaplasma TK | 9.9 ± 5.2 | 0.055 ± 0.008 | 5.6 ± 1.5 |
- *Assays were performed using phosphoryl transfer assay with [γ-32P]-labelled ATP (100 μM) and variable concentrations of TFT (1 – 100 μM). The reaction products were separated by thin layer chromatography and were quantified. Thymidine (10 μM) was used as a control. Data are from three independent measurements and are expressed as mean ± SD.