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Table 4 Kinetic parameters of trifluorothymidine with purified recombinant human TK1, TK2, and Ureaplasma TK*

From: Inhibition of Mycoplasma pneumoniae growth by FDA-approved anticancer and antiviral nucleoside and nucleobase analogs

  Km(μM) kcat(s-1) kcat/Km(s-1M-1)×103
Human TK1 5.9 ± 1.7 0.043 ± 0.003 7.3 ± 1.8
Human TK2 8.8 ± 3.8 0.026 ± 0.003 3.0 ± 0.8
Ureaplasma TK 9.9 ± 5.2 0.055 ± 0.008 5.6 ± 1.5
  1. *Assays were performed using phosphoryl transfer assay with [γ-32P]-labelled ATP (100 μM) and variable concentrations of TFT (1 – 100 μM). The reaction products were separated by thin layer chromatography and were quantified. Thymidine (10 μM) was used as a control. Data are from three independent measurements and are expressed as mean ± SD.