Table 1 Bacterial strains and plasmids used for this study
From: OmpR and RcsB abolish temporal and spatial changes in expression of flhD in Escherichia coli Biofilm
Strains | Relevant genotypes | Reference |
|---|---|---|
AJW678 | thi-1 thr-1(am) leuB6 metF159(Am) rpsL136 ΔlaxX74 | [57] |
AJW2050 | AJW678 ompR::Tn10 | [42] |
AJW2143 | AJW678 rcsB::Tn 5 | [60] |
MC1000 | F-, araD 139 Δ(araAB leu)7696 Δ(lacX74) galU galK strA prsL thi | [59] |
BP1470 | AJW678 pPS71 | This study |
BP1531 | AJW2050 pPS71 | This study |
BP1532 | AJW2143 pKK12 | This study |
BP1432 | AJW678 ompR::gfp | This study |
BP1462 | AJW678 pEC2 | This study |
BP1437 | AJW678 aceK::gfp | This study |
Plasmids | ||
pPS71 | pUA66 flhD::gfp | This study |
pKK12 | pPS71 CmR | This study |
pOmpR::gfp | pUA66 ompR::gfp | [62] |
pEC2 | pAcGFP rcsB::gfp | This study |
pAceK::gfp | pUA66 aceK::gfp | [62] |