Figure 3

Close-up views of regions targeted by site-directed mutagenesis. The structures of PAS domains used to select the residues to replace are shown on the left (A,C,E), and the corresponding views of the PAS_Bvg model are shown on the right (B,D,F). The residues that were replaced are colored yellow. A, FixL_Bj PAS domain (pdb code: 1DRM), with the heme colored grey. C, PAS domain of the M. tuberculosis Rv1364c protein (pdb code: 3KC3), showing the fatty acid in the cavity (in grey). E, cavity of PAS_Hm (pdb code: 3BWL) with the Asp side chains (in yellow) pointing to the 1H-indole-3 carbaldehyde ligand (in grey). In PAS_Bvg(F) the corresponding residues are Tyr₅₉₆ and Asn₆₃₁.