Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO2 fixation into poly (3-hydroxybutyrate) under heterotrophic condition in Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses

Table 1 Summary of RNA-sequencing

Sample	Run	F16	F26	F36	O26
C-source, cultivation time		Fructose, 16 h	Fructose, 26 h	Fructose, 36 h	Octanoate, 26 h
Cellular phase		Growth	PHA production	Stationary	PHA production
Number of reads	1	38,214,032	39,930,488	33,340,159	26,442,674
	2	41,400,577	40,262,640	28,544,022	40,339,722
Reads on rRNA regions^a	1	27,521,315	34,573,643	27,787,869	23,463,979
	2	30,737,637	34,721,392	23,478,331	35,483,825
Reads on CDSs other than rRNA regions^a (Total reads)	1	10,692,717	5,356,845	5,552,290	2,978,695
	2	10,662,940	5,541,248	5,065,691	4,855,897

^aH16_A1659-A1661 (rrsA-rrlA-rrfA), H16_A3306-A3302 (rrsB-tRNA[Ile]-tRNA[Ala]-rrlB-rrfB), and H16_A3513-A3509 (rrsC-tRNA[Ile]-tRNA[Ala]-rrlC-rrfC) on chromosome 1, and H16_B0151-B0155 (rrsD-tRNA[Ile]-tRNA[Ala]-rrlD-rrfD) and H16_B0769-B0773 (rrsE-tRNA[Ile]-tRNA[Ala]-rrlE-rrfE) on chromosome 2.

ISSN: 1471-2180