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Table 1 Cell density, unronic acid (alginate) content and extracellular lipase activity of agar-grown P. aeruginosa biofilms

From: Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa

Strain

Bacterial density × 109(cells/cm2)

Uronic acids (μg/cm2)

Lipase activity (nmol/min x cm2)

SG81

1.4 ± 0.3

0.22 ± 0.01

0.12 ± 0.01

SG81MCS

1.3 ± 0.2

0.23 ± 0.01

0.14 ± 0.01

SG81ΔlipA

1.2 ± 0.1

0.22 ± 0.01

0.0 ± 0.0

SG81ΔlipA::lipA

1.5 ± 0.6

0.23 ± 0.03

6.50 ± 0.1

SG81lipA+

1.4 ± 0.2

0.23 ± 0.01

63.02 ± 5.2

  1. The mucoid parent strain P. aeruginosa SG81 and its derivative strains (vector control SG81MCS, lipA mutant SG81ΔlipA, complementation strain SG81ΔlipA::lipA and lipA overexpression strain SG81lipA+) were tested. The results are expressed as mean values of four independent experiments.
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BMC Microbiology

ISSN: 1471-2180

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