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Table 3 PFGE and fAFLP typing results from a panel of 97 L. monocytogenes isolates with index of discrimination (ID)

From: Fluorescence amplified fragment length polymorphism compared to pulsed field gel electrophoresis for Listeria monocytogenes subtyping

L. monocytogeneslineages Serogroups1or serotype2 Number of isolates Number of PFGE3types PFGE ID4 Number of fAFLP3types fAFLP ID4
I IVb 35 36 0.988 33 0.981
IIb 11
II IIa 45 45 0.995 43 0.989
IIc 5
III 4a 1 1 n/a 1 n/a
Total: 97 82 0.996 76 0.993
  1. 1 Serogrouping performed by multiplex PCR [4]: results are from both the European Reference Laboratory (EURL) for L. monocytogenes and the UK National Reference laboratory (UK-NRL) for Listeria.
  2. 2 Based on sero-agglutination performed by EURL.
  3. 3ApaI/AscI-PFGE was performed by the EURL and HindIII/HhaI-fAFLP by UK-NRL.
  4. 4 Index of discrimination calculated according to the Simpson’s index of diversity (ID) [25]. n/a: not applicable.