Table 5 Bacterial strains and plasmids used in this study
From: Adaptive mechanisms of Campylobacter jejunito erythromycin treatment
Strain or plasmid | Description or relevant genotype | Source or reference |
|---|---|---|
Bacterial strains | ||
NCTC 11168 | Campylobacter jejuni wild-type strain | ATCC* |
JL272 | NCTC 11168 derivative erythromycin resistant strain | Jun Lin [20] |
KOp50Q | NCTC 11168 Δcj1169c-cj1170c::cat mutant | This study |
KO423Q | NCTC 11168 Δcj0423-cj0425::aphA3 mutant | This study |
KO39Q | NCTC 11168 Δcj0309c-cj0310c::cat mutant | This study |
KO73Q | NCTC 11168 Δcj1173-cj1174::aphA3 mutant | This study |
DKO01Q | NCTC 11168 Δcj0309c-cj0310::cat, Δcj1173-cj1174::aphA3 mutant | This study |
Comp50Q | KOp50Q complementation strain; Δcj1169c-cj1170c::cat, ITS1::cj1169c-cj1170c complementation strain | This study |
ComDK01Q | DKO01Q complementation strain; Δcj0309c-cj0310::cat, Δcj1173-cj1174::aphA3, ITS1::cj1173-cj1174 | This study |
DH5α | Escherichia coli strain for plasmid propagation | Invitrogen |
Plasmids | ||
pUC19 | Polymerase chain reaction cloning vector, AmpR** | Promega |
pRR | Complementation cloning vector | Karlyshev [40] |
pRRK | pRR with aphA3; KanR** | This study |
pRRT | pRR with tetO; TetR** | This study |
pUp50Q | cj1169c-cj1170c::cat cloned into pUC19 | This study |
pU423Q | cj0423-cj0425::aphA3 cloned into pUC19 | This study |
pU39Q | cj0309c-cj0310c::cat cloned into pUC19 | This study |
pU74Q | cj1173-cj1174::aphA3 cloned into pUC19 | This study |
pRRKp50Q | cj1169c-cj1170c cloned into pRRK | This study |
pRRK39Q | cj0309c-cj0310c cloned into pRRK | This study |
pRRT73Q | cj1173-cj1174 cloned into pRRT | This study |