E. coli W secretes SslE using T2SS
in a condition-dependent manner. All lanes are labeled by sample type: C = cell lysate, S = culture supernatant, M = molecular weight standards. A. Lysates and concentrated cell-free supernatants of wild-type and Δgsp strains showing SslE secretion by T2SSβ. B. Complementation of the ΔsslE mutation: WT = wild-type, VOC = vector-only control, SslE-SP = SslE lacking an N-terminal signal peptide. C. Complementation of the ΔpppA mutation. D. Condition-dependence of SslE secretion labeled by temperature and growth medium. Sizes of molecular weight standards are shown to the side of each gel in kDa. The presence of secreted SslE is marked with black triangles.