Table 1 Libraries description and construction method.
From: Host gene response to endosymbiont and pathogen in the cereal weevil Sitophilus oryzae
| Â | Library | Type | Origin | Status of infection | Presence of symbiont | Description | Number of individuals / bacteriomes sampled and pooled (quantity of RNA used from samples) |
|---|---|---|---|---|---|---|---|
Host response to pathogen | SSH1 | Subtraction | Whole larvae | infected | no | Salmonella+ vs. Salmonella- | Salmonella -: 10 uninfected aposymbiotic larvae (10µg) |
|  | SSH2 | Subtraction | Whole larvae | Not infected | no | Salmonella- vs. Salmonella+ | Salmonella +: 15 infected aposymbiotic larvae: 5 collected 3h after infection (3.33µg), 5 after 6h (3.33µg) and 5 after 12h (3.33µg) |
Host response to symbiont | SSHA | Subtraction | Bacteriome | Not infected | yes | With symbiont vs. without symbiont | With symbiont: 200 symbiotic bacteriomes (10 µg) |
|  | SSHB | Subtraction | Bacteriome | Not infected | no | Without symbiont vs. with symbiont | Without symbiont: 640 aposymbiotic bacteriomes (10 µg) |
|  | SO | Non-normalized | Bacteriome | Not infected | yes | Pool of bacteriomes with symbiont | 170 symbiotic bacteriomes (10µg) |
|  | AO | Non-normalized | Bacteriome | Not infected | no | Pool of bacteriomes without symbiont | 578 aposymbiotic bacteriomes (10 µg) |
|  | NOR | Normalized | Whole larvae | infected | yes | Pool of Symbiotic Larvae / Aposymbiotic larvae / Aposymbiotic larvae infected during 3h, 6h and 12h | 10 uninfected aposymbiotic larvae (2µg) / 10 uninfected symbiotic larvae (2µg) / 15 infected aposymbiotic larvae: 5 collected after 3h of infection (2µg), 5 after 6h (2µg) and 5 after 12h (2µg) |