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Figure 2 | BMC Microbiology

Figure 2

From: Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients

Figure 2

Discrepancy between direct sequencing and pyrosequencing in sample NN124. The direct sequencing method (A) detected the nucleotides (GTG) coding for the rtM204V variant, although the electropherogram indicated mixtures with small quantities of nucleotides A and T corresponding to the first and third nucleotide position of codon ATT (rt204I). Pyrosequencing (B) detected about 60% YIDD (I/ATT) and 40% YVDD (V/GTG) variants

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