Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour

Table 2 Correlation of gene expression data (fold changes) obtained by DNA-microarray and real time qPCR

MAP acid-nitrosative stress transcriptome
Gene ID	Gene name	Gene Product	Microarray fold change	P-value	Real Time-qPCR fold change	SD
*MAP 3738c*	-	Methyltransferase type 12 / Cyclopropane-fatty-acyl-phospholipid synthase	-2.78465771	0.00216317	-2.89367248	0.17
*MAP 3522*	oxyS	Transcriptional regulator, oxyS	4.02084912	0.00065264	2.66363166	0.60
*MAP 1643*	aceAb	Isocitrate lyase	7.02500864	0.00052984	4.30330061	0.07
MAP THP-1 infection transcriptome
Gene ID	Gene name	Gene Product	Microarray fold change	P-value	Real Time-qPCR fold change	SD
*MAP 0654*	phoT	Phosphate transporter ATP-binding protein	-42.44433187	0.02392446	-16.81349291	0.91
*MAP 1407*	-	ADP-ribose pyrophosphatase	69.43061281	0.04255943	27.68837536	0.74
*MAP 1317c*	-	Acid-resistance membrane protein	4.39998925	0.00351578	2.90831542	2.42
*MAP 1535*	pgsA2	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	6.40855813	0.00166329	2.51498937	6.99
*MAP 2055*	-	Cystathione beta-lyase	-9.04737958	0.00004972	-36.48386353	0.64

Selected MAP genes were validated for their expression profile by Real-Time qPCR to corroborate similar results in microarray data. Three selected genes are shown for the MAP acid-nitrosative stress transcriptome whereas five genes are shown for MAP THP-1 infection transcriptome. Gene ID: Gene identification code; SD: Standard deviation.

ISSN: 1471-2180