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Table 2 Correlation of gene expression data (fold changes) obtained by DNA-microarray and real time qPCR

From: Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour

MAP acid-nitrosative stress transcriptome
Gene ID Gene name Gene Product Microarray fold change P-value Real Time-qPCR fold change SD
MAP 3738c - Methyltransferase type 12 / Cyclopropane-fatty-acyl-phospholipid synthase -2.78465771 0.00216317 -2.89367248 0.17
MAP 3522 oxyS Transcriptional regulator, oxyS 4.02084912 0.00065264 2.66363166 0.60
MAP 1643 aceAb Isocitrate lyase 7.02500864 0.00052984 4.30330061 0.07
MAP THP-1 infection transcriptome
Gene ID Gene name Gene Product Microarray fold change P-value Real Time-qPCR fold change SD
MAP 0654 phoT Phosphate transporter ATP-binding protein -42.44433187 0.02392446 -16.81349291 0.91
MAP 1407 - ADP-ribose pyrophosphatase 69.43061281 0.04255943 27.68837536 0.74
MAP 1317c - Acid-resistance membrane protein 4.39998925 0.00351578 2.90831542 2.42
MAP 1535 pgsA2 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 6.40855813 0.00166329 2.51498937 6.99
MAP 2055 - Cystathione beta-lyase -9.04737958 0.00004972 -36.48386353 0.64
  1. Selected MAP genes were validated for their expression profile by Real-Time qPCR to corroborate similar results in microarray data. Three selected genes are shown for the MAP acid-nitrosative stress transcriptome whereas five genes are shown for MAP THP-1 infection transcriptome. Gene ID: Gene identification code; SD: Standard deviation.