A Fur-like protein PerR regulates two oxidative stress response related operons dpr and metQIN in Streptococcus suis

Table 3 Strains and plasmids used in this study

Strains or plasmids	Characteristics	Reference or source
Strains
SC-19	Virulent Chinese S. suis serotype 2 isolate, wild-type	This work
Δ perR	Gene perR inactive strain, Erm^r	This work
CΔ perR	Complemented Δ perR strain, Erm^r Spc^r	This work
Δ dpr	Gene dpr inactive strain, Spc^r	This work
Δ perRΔdpr	Gene perR and dpr inactive strain, Erm^r Spc^r	This work
SC-19:EGFP	P_dpr-EGFP fusion inserted Wild-type, Spc^r	This work
Δ perR:EGFP	P_dpr-EGFP fusion inserted Δ perR, Spc^r	This work
DH5αand BL21(DE3)	Cloning and expression host	In this lab
Plasmids
pSET4s	Thermosensitive allelic replacement vector	[42]
pSET2	E. coli-S. suis shuttle vector	[44]
pET28a	His tag fusion expression vector	Novagen
pMIDG310	A plasmid containing a EGFP gene
pSET4s:: perR	A mosaic plasmid designed to inactivate perR	This work
pSET4s::dpr	A mosaic plasmid designed to inactivate dpr	This work
pSET2::CperR	Recombinant plasmid used for functional complementation of ΔperR	This work
pSET4s:EGFP	Recombinant plasmid used for inserting a P_dpr-EGFP fusion	This work
pET28a:: perR	Recombinant expression plasmid to produce His₆-fused PerR protein	This work
pAT18	A plasmid containing an erm	[45]

ISSN: 1471-2180