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Figure 5 | BMC Microbiology

Figure 5

From: LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situhybridization

Figure 5

Comparison between LNA and DNA probes for detecting endosymbiont of lower abundance ( Arsenophonus ). All specimens were processed using the procedure described for Portiera. However, the probe concentration used for Arsenophonus was 1.0 pmoles and kept identical for LNA and DNA. This graph depicts the signal intensity profiles of LNA and DNA probes as a function of formamide concentration after background subtraction. At the same concentration, the intensity profile of LNA probe is significantly higher than the DNA probe while detecting Arsenophonus, an endosymbiont of low abundance. Fluorescence intensities were quantified by NIS elements (V 3.21.02) image analysis software (Nikon).

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BMC Microbiology

ISSN: 1471-2180

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