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Table 1 Influence of a single mismatch near the 3′ end in the RDP dataset

From: Coverage evaluation of universal bacterial primers using the metagenomic datasets

Primer Phylum Non-coverage rate 4+ (%) Non-coverage rate 4- (%)
338 F Lentisphaerae 3.0 100.0
  OP3 5.9 100.0
  Chlamydiae 33.5 99.6
  OD1 36.3 97.8
  Planctomycetes 71.9 98.9
519 F Nitrospirae 3.0 68.1
  Spirochaetes 1.2 63.3
  Chloroflexi 1.5 59.2
  Planctomycetes 3.4 59.1
  Thermotogae 0.0 54.6
  WS3 2.4 43.4
  OP10 0.0 29.8
  OP8 0.7 21.7
  Cyanobacteria 0.6 21.3
  Gemmatimonadetes 0.6 20.7
  Unclassified Bacteria 2.4 28.4
  1. At the phylum level, non-coverage rates that changed more than 20% under two criteria are listed. “Non-coverage rate 4+” denotes the non-coverage rate when a single mismatch in the last 4 nucleotides was allowed. “Non-coverage rate 4-” denotes the non-coverage rate when mismatches in the last 4 nucleotides were not allowed.