Figure 1

Transcriptional organization of the bioYMN locus in C. glutamicum. (upper panel) Scheme showing the bioYMN locus in C. glutamicum and the RT-PCR reactions used to determine co-transcription of bioY, bioM and bioN. RNA from C. glutamicum WT was transcribed into cDNA with random primers. Subsequently, cDNAs were used as templates for the PCR reactions labeled A-C. (middle panel) Results from the RT-PCR analyses described above. The lower DNA fragment visible lanes A-C represents dnaE, and RT-PCR of dnaE served as positive control in all reactions. The upper bands in lanes A, B and C correspond to the products of the PCR reactions A-C indicated in A. Reactions AN, BN and CN represent controls confirming the absence of DNA in the RNA preparation. The reactions were identical to the PCR reactions as shown in lanes A-C except that reverse transcriptase was omitted in the cDNA reactions. (lower panel) The bioYMN locus is shown schematically. The translational start codon of bioY is boxed, the transcriptional start site of bioYMN is marked as +1, the -10 and -35 promoter hexamers are given in grey and marked below the cg2146-bioY intergenic sequence and are compared to the consensus sequences described in ref. [30], which are depicted above the cg2146-bioY intergenic sequence. The translational stop codon of bioN and the bioN-cg2151 intergenic sequence is depicted with a potential transcriptional termination signal rendered in grey and highlighted by arrows above the bioN-cg2151 intergenic sequence.