Table 4 Laboratory quantitative validation results of the BactQuant assay performed using pure plasmid standards and different mixed templates
From: BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
Templates used | Assay dynamic range | Average reaction efficiency (SD) | r2–value |
|---|---|---|---|
Plasmid standards–only (10 μl Rxn) | 100–108 copies | 102% (2%) | >0.999 |
Plasmid standards-only (5 μl Rxn) | 100 – 108 copies | 95% (1%) | >0.999 |
Plasmid standards plus 0.5 ng human gDNA | 100 – 108 copies | 99% (4%) | >0.994 |
Plasmid standards plus 1 ng human gDNA | 100 – 108 copies | 101% (5%) | >0.994 |
Plasmid standards plus 5 ng human gDNA | 500 – 108 copies | 96% (1%) | >0.999 |
Plasmid standards plus 10 ng human gDNA | 1000 – 108 copies | 97% (2%) | >0.999 |
Plasmid standards plus 0.5 ng C. albicans gDNA | 100 – 108 copies | 97% (1%) | >0.999 |