Skip to main content

Table 4 Laboratory quantitative validation results of the BactQuant assay performed using pure plasmid standards and different mixed templates

From: BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay

Templates used Assay dynamic range Average reaction efficiency (SD) r2–value
Plasmid standards–only (10 μl Rxn) 100–108 copies 102% (2%) >0.999
Plasmid standards-only (5 μl Rxn) 100 – 108 copies 95% (1%) >0.999
Plasmid standards plus 0.5 ng human gDNA 100 – 108 copies 99% (4%) >0.994
Plasmid standards plus 1 ng human gDNA 100 – 108 copies 101% (5%) >0.994
Plasmid standards plus 5 ng human gDNA 500 – 108 copies 96% (1%) >0.999
Plasmid standards plus 10 ng human gDNA 1000 – 108 copies 97% (2%) >0.999
Plasmid standards plus 0.5 ngC. albicans gDNA 100 – 108 copies 97% (1%) >0.999