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Table 3 The efficiency and r 2 -value results from laboratory evaluation of the BactQuant assay using genomic DNA from ATCC strains and clinical isolates belonging to 106 unique bacterial species spanning eight bacterial phyla

From: BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay

Species Name Reaction efficiency r2-value
Streptomyces violaceoruber 93% >0.999
Mycobacterium abscessus 110% >0.999
Mycobacterium bovis 106% >0.996
Mycobacterium chelonae 101% >0.999
Mycobacterium gastri 104% >0.999
Mycobacterium gordonae 104% >0.999
Mycobacterium fortuitum 93% >0.999
Mycobacterium kansasii 107% >0.999
Mycobacterium marinum 110% >0.990
Mycobacterium nonchromogenicum 101% >0.999
Mycobacterium phlei 104% >0.999
Mycobacterium smegmatis 105% >0.999
Mycobacterium vaccae 120% >0.999
Mycobacterium xenopi 112% >0.999
Bacteroides ureolyticus 92% >0.999
Bacteroides fragilis 82% >0.993
Chlamydia trachomatis N/A N/A
Chlamydophila pneumoniae N/A N/A
Thermus thermophilus 97% >0.999
Clostridium difficile 88% >0.987
Listeria monocytogenes 104% >0.999
Staphylococcus arlettae 96% >0.998
Staphylococcus capitis 95% >0.993
Staphylococcus cohnii 104% >0.999
Staphylococcus epidermidis 96% >0.999
Staphylococcus equorum 85% >0.997
Staphylococcus hominis 108% >0.999
Staphylococcus haemolyticus 90–104% >0.999
Staphylococcus kloosii 98% >0.999
Staphylococcus lugdunensis 94% >0.999
Staphylococcus saprophyticus 87–98% >0.999
Staphylococcus xylosus 81–100% >0.999
Streptococcus agalactiae 98% >0.998
Streptococcus pneumoniae 98% >0.999
Streptococcus viridans 103% >0.999
Enterococcus faecium 91–111% >0.999
Enterococcus faecalis 90–100% >0.998
Fusobacterium nucleatum 90% >0.999
Burkholderia pseudomallei 103% >0.999
Coxiella burnetti* 100% >0.998
Francisella tularensis 100% >0.999
Legionella pneumophila 98% >0.999
Neisseria gonorrhoeae 95% >0.997
Pseudomonas aeruginosa 90–100% >0.999
Pseudomonas mendocina 93% >0.999
Pseudomonas andersonii 90% >0.999
Pseudomonas otitidis 93% >0.999
Pseudomonas stutzeri 86% >0.999
Pseudomonas monteilii 88% >0.999
Pseudomonas azotofixans 84% >0.999
Pseudomonas mosselii 92% >0.999
Pseudomonas luteola 91% >0.999
Pseudomonas putida 90% >0.999
Pseudomonas fluorescens 96% >0.999
Pseudomonas taetrolens 89% >0.999
Pseudomonas fragi 93% >0.999
Pseudomonas syringae 95% >0.999
Pseudomonas pseudoalcaligenes 93% >0.999
Pseudomonas lundensis 93% >0.999
Pseudomonas anguiliseptica 93% >0.999
Cellvibrio gilvus 92% >0.999
Acinetobacter baumannii 100–105% >0.999
Arsenophonus nasoniae 87% >0.998
Budvicia aquatica 88% >0.999
Buttiauxella gaviniae 107% >0.999
Cedecea davisae 97% >0.999
Citrobacter freundii 95% >0.999
Cronobacter sakazakii 96% >0.999
Edwardsiella tarda 106% >0.999
Enterobacter cloacae 89–111% >0.999
Enterobacter aerogenes 107% >0.998
Escherichia vulneris 93% >0.999
Escherichia coli 91–96% >0.999
Ewingella americana 97% >0.999
Haemophilus influenzae 91–110% >0.999
Hafnia alvei 93% >0.999
Klebsiella oxytoca 93% >0.999
Klebsiella pneumoniae 95–100% >0.999
Kluyvera ascorbata 100% >0.999
Leclercia adecarboxylata 93% >0.999
Leminorella richardii 94% >0.999
Moellerella wisconsensis 93% >0.999
Moraxella catarrhalis 91–106% >0.999
Morganella morganii 95% >0.999
Obesumbacterium proteus 114% >0.994
Pantoea agglomerans 93% >0.999
Pectobacterium atrosepticum 90% >0.999
Photorhabdus asymbiotica 96% >0.999
Plesiomonas shigelloides 93% >0.999
Pragia fontium 100% >0.998
Proteus mirabilis 98% >0.999
Providencia rustigianii 93% >0.999
Rahnella aquatilis 92% >0.999
Raoultella ornithinolytica 94% >0.999
Salmonella enterica 101% >0.999
Salmonella enterica subsp. enterica serovar gallinarum 95% >0.998
Serratia liquefaciens 94% >0.999
Shigella dysenteriae 98% >0.999
Tatumella ptyseos 101% >0.999
Trabulsiella guamensis 95% >0.999
Yokenella regensburgei 96% >0.999
Yersinia enterocolitica 98% >0.999
Campylobacter jejuni 89% >0.999
Vibrio cholerae 85% >0.996
Borrelia burgdorferi 90% >0.999
Treponema denticola 82% >0.999
  1. *No 16 S rRNA gene sequence available in the Ribosomal Database Project.