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Table 1 Identification of the seroreactive proteins of C. burnetii by MALDI-TOF-MS and ESI-MS/MS

From: Potential serodiagnostic markers for Q fever identified in Coxiella burnetiiby immunoproteomic and protein microarray approaches

spot no Identification Gene name Locus tag NCBI no. Nominal mass Calculated pI value Identify method Score Expect value Queries matched %Sequence coverage Mice sera (-days-p.i.) Human sera(A,B)
1 Chaperone protein dnaK CBU_1290 gi|29654590 70826 5.14 MALDI-TOF 176 6.80E-12 21 38% - A,B
2 Chaperonin GroEL groEL CBU_1718 gi|161830449 58375 5.14 MALDI-TOF 200 2.70E-14 24 52% 14,21,28 A,B
3 Trigger factor tig CBU_0737 COXBURSA gi|29654071 50215 5.3 MALDI-TOF 223 1.40E-16 32 67% 28 A,B
4 F0F1 ATP synthase subunit beta atpD 331_A2148 gi|161830152 50490 5.01 MALDI-TOF 240 2.70E-18 26 54% 21,28 A,B
5 Dihydrolipoyllysine-residue succinyltransferase sucB CBU_1398 gi|29654691 45908 5.54 MALDI-TOF 100 0.00027 16 34% 21,28 A
6 Fructose-1,6-bisphosphate aldolase fbaA CBU_1778 gi|29655066 39793 5.41 MALDI-TOF 190 2.70E-13 16 48% 21,28 A,B
7 S-adenosylmethionine Synthetase metK CBU_2030 gi|29655311 43150 5.55 MALDI-TOF 153 1.40E-09 20 50% - A,B
8 3-oxoacyl-[acyl-carrier-protein] synthase 2 fabF CBU_0497 gi|29653839 44275 5.49 MALDI-TOF 160 2.70E-10 20 58% - A
9 Elongation factor Tu tuf2 CBU_0236 gi|29653588 43613 5.32 MALDI-TOF 285 8.60E-23 29 76% 28 A,B
10 Glutamine synthetase glnA CBU_0503 gi|29653845 39876 5.33 MALDI-TOF 122 1.7e-06 15 44% - A
11 Malate dehydrogenase mdh CBU_1241 gi|29654544 35732 5.07 MALDI-TOF 136 6.80E-08 19 50% 21,28 A
12 34 kDa outer membrane protein ybgF - gi|30025849 33641 5.67 MALDI-TOF 92 0.0019 8 28% 21,28 A
13 (2R)-phospho-3-sulfolactate synthase comA CBU_1954 gi|29655237 33383 5.38 MALDI-TOF 146 6.80E-09 16 52% 28 A
14 Inorganic diphosphatase ppa CBU_0628 gi|29653966 19642 5.2 ESI-MS/MS 323 2.1e-26 7 36% 28 -
15 LSU ribosomal protein L12P (L7/L12) rplL CBU_0229 COXBURSA gi|29653581 13240 4.71 ESI-MS/MS 210 4.2e-15 6 48% - A,B
16 30S ribosomal protein S2 rpsB 331_A1545 gi|161831161 35410 8.88 MALDI-TOF 100 0.00027 15 48% 28 -
17 Peptidyl-prolyl cis-trans isomerase Mip mip CBU_0630 gi|29653968 25501 9.8 MALDI-TOF 133 6.10E-07 9 57% 14,21,28 -
18 27 kDa outer membrane protein com1 - gi|11935138 26739 9.23 MALDI-TOF 95 0.00078 7 42% 14,21,28 -
19 Acute disease antigen A adaA CBU_0952 gi|29654269 25935 8.67 MALDI-TOF 110 2.70E-05 15 38% - B
20 Putative outer membrane Skp ompH CBU_0612 gi|29653950 18812 9.71 ESI-MS/MS 429 4.3e-37 5 28% 14,21,28 -