Purification of LysB4 and lytic activity of LysB4. (a) Purified LysB4 was loaded on an SDS-PAGE gel. Lane M, molecular weight marker; lane 1, the purified LysB4 fraction. (b) Different concentration of LysB4 was added to the suspension of B.cereus ATCC 10876, and decrease in turbidity was monitored. (c) Diluted suspension of B. cereus ATCC10876 (100 μl) was mixed with 5 μg of LysB4 and observed under optical microscope (× 1,000 magnification).