Figure 3From: The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citriGpsX is involved in polysaccharide biosynthesis in X. citri subsp. citri. (A) EPS production in NB medium supplemented with 2% (w/v) glucose by wild type strain 306 and its derivatives. The data presented are the means ± SD of triplicate measurements from a representative experiment; similar results were obtained in two other independent experiments. (B) Analysis of LPS synthesis. The LPSs produced by wild type strain 306 and its derivatives were extracted, subjected to SDS-PAGE analysis, and visualized by silver staining. The lost bands in the mutants are indicated by arrows. WT: wild-type strain 306; M: gpsX mutant C223 G4 (gpsX-); MV: gpsX mutant 223G4V (gpsX-) with empty vector pUFR053; CM: complemented gpsX mutant C223 G4 (gpsX+); S: LPS standard from S. enterica serovar Typhimurium (10 μg; Sigma). The experiments were repeated three times with similar results, and the results of only one experiment are presented.Back to article page