GD1-fed worms are more resistant to juglone treatment and show enhanced thermotolerance. (A) Wild-type N2 worms were fed OP50 or GD1 from the hatchling stage. L4 larval worms were placed in a drop of S-media containing either 250 μM juglone or an equal amount of ethanol vehicle control for 20 min. Worms were washed onto OP50 plates to recover and assayed for survival 18 h later. Black bar: OP50, grey bar: GD1; Asterisk indicates p-value = 0.0003 determined with Student’s t-test when compared to the OP50 + juglone condition. (B) Wild-type N2 worms were fed OP50 or GD1 from the hatchling stage. L4 larval worms were incubated at 35°C and survival was assessed at each indicated time point. Black line: OP50, grey line: GD1. Asterisks indicate p-values determined with Student’s t-test for comparisons between GD1 and OP50 at the designated time points: (7 h) 0.003; (9 h) 0.0013; (10 h) 0.0001; (11 h) 0.017.