Skip to main content

Advertisement

Table 1 Strains and plasmids

From: Quorum sensing modulates colony morphology through alkyl quinolones in Pseudomonas aeruginosa

Strain or plasmid Strain Relevant property Reference
P. aeruginosa   
PA14 Wild-type [39]
PAO1 Wild-type [40]
ZK2870 Wild-type [12]
PAO1 lasR Markerless lasR mutant derived from PAO1 [41]
PA14 lasR TnphoA lasR mutant derived from PA14 [42]
ZK lasR Markerless in-frame lasR deletion in ZK2870 This study
ZK pelA Markerless pelA deletion in ZK2870 [11]
ZK pslD Markerless pslD deletion in ZK2870 [11]
ZK lasI Markerless lasI deletion in ZK2870 This study
ZK pelA lasR Markerless lasR deletion in a pelA mutant of ZK2870 This study
ZK pslD lasR Markerless lasR deletion in a pslD mutant of ZK2870 This study
ZK pqsH Markerless pqsH deletion in ZK2870 This study
ZK tpbA Markerless pqsH deletion in ZK2870 This study
ZK lasR pqsA suppressor mutation in a lasR mutant of ZK2870 This study
pqsA::Tn   
ZK lasR pqsR suppressor mutation in a lasR mutant of ZK2870 This study
pqsR::Tn   
E. coli   
DH5α F- φ80dlacZΔM15 Δ (lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK- mK+) phoA supE44 λ-thi-1 gyrA96 relA1 Invitrogen
SM10 Mobilizing strain, RP4 tra genes integrated in the chromosome, thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr [43]
S17-1/λpir TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir [43]
Plasmid   
mini-CTX-lacZ Chromosomal integration vector, TcR [44]
pEX18 Tc Allelic exchange suicide vector, TcR [45]
pEX18.ΔlasR Suicide vector with lasR in-frame deletion [41]
pEX18.ΔlasI Suicide vector with lasI in-frame deletion [41]
pEX18.ΔtpbA Suicide vector containing tpbA in-frame deletion This study
pLM1 Tn5 delivery vector, GmR [46]
pLG10 pqsA-E operon cloned in pUCP18, ApR [24]
pRG10 pqsA-D operon cloned under control of P lac of pUCP18, ApR This study
pRG11 Promoter region of pel cloned in mini-CTX-lacZ vector This study
pUCP18 Parent vector of pLG10, ApR [47]