Table 1 Strains and plasmids

P. aeruginosa

PA14

Wild-type

[39]

PAO1

Wild-type

[40]

ZK2870

Wild-type

[12]

PAO1 lasR

Markerless lasR mutant derived from PAO1

[41]

PA14 lasR

TnphoA lasR mutant derived from PA14

[42]

ZK lasR

Markerless in-frame lasR deletion in ZK2870

This study

ZK pelA

Markerless pelA deletion in ZK2870

[11]

ZK pslD

Markerless pslD deletion in ZK2870

[11]

ZK lasI

Markerless lasI deletion in ZK2870

This study

ZK pelA lasR

Markerless lasR deletion in a pelA mutant of ZK2870

This study

ZK pslD lasR

Markerless lasR deletion in a pslD mutant of ZK2870

This study

ZK pqsH

Markerless pqsH deletion in ZK2870

This study

ZK tpbA

Markerless pqsH deletion in ZK2870

This study

ZK lasR

pqsA suppressor mutation in a lasR mutant of ZK2870

This study

pqsA::Tn

ZK lasR

pqsR suppressor mutation in a lasR mutant of ZK2870

This study

pqsR::Tn

E. coli

DH5α

F^- φ80dlacZΔM15 Δ (lacZYA-argF) U169 deoR recA1 endA1 hsdR17(r_K^- m_K⁺) phoA supE44 λ^-thi-1 gyrA96 relA1

Invitrogen

SM10

Mobilizing strain, RP4 tra genes integrated in the chromosome, thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km^r

[43]

S17-1/λpir

Tp^R Sm^R recA, thi, pro, hsdR-M⁺RP4: 2-Tc:Mu: Km Tn7 λpir

[43]

Plasmid

mini-CTX-lacZ

Chromosomal integration vector, Tc^R

[44]

pEX18 Tc

Allelic exchange suicide vector, Tc^R

[45]

pEX18.ΔlasR

Suicide vector with lasR in-frame deletion

[41]

pEX18.ΔlasI

Suicide vector with lasI in-frame deletion

[41]

pEX18.ΔtpbA

Suicide vector containing tpbA in-frame deletion

This study

pLM1

Tn5 delivery vector, Gm^R

[46]

pLG10

pqsA-E operon cloned in pUCP18, Ap^R

[24]

pRG10

pqsA-D operon cloned under control of P _lac of pUCP18, Ap^R

This study

pRG11

Promoter region of pel cloned in mini-CTX-lacZ vector

This study

pUCP18

Parent vector of pLG10, Ap^R

[47]