YFP fluorescence of Drosophila S2 cells expressing fusion proteins. A) cells expressing DynA-YFP early after induction, or B) 6 hours after induction. Shown are planes in the middle of cells, C) S2 cells expressing FloT-YFP, shown is the middle plane or the surface of the cells, as indicated by the lines within the circle. D) Non-transfected cells, the outline can be seen in the bright field channel; membrane stain also shows the outline of cells, but the membrane cannot be distinguished from the background of the cell; panel “YFP” shows background fluorescence in non-transfected cells in the YFP channel. White or grey bars 2 μm.