Table 1 Oligonucleotide primers pairs used in this study
From: Characteristics of a broad lytic spectrum endolysin from phage BtCS33 of Bacillus thuringiensis
Primer pairs | Sequence (5'-3') | PCR products (Size) | Predicted products/Size (amino acid residues) |
|---|---|---|---|
plyBt33-F/ BamHI | GAGGATCC*ATGGGTTACACTGTAGATATTTC | plyBt33 (816bp) | PlyBt33/33kDa (amino acid residues 1–272) |
plyBt33-R/ SalI | GACGTCGACTTCTTTTGTATAAAAGTATTTAA | Â | Â |
plyBt33-F/ BamHI | GAGGATCCATGGGTTACACTGTAGATATTTC | plyBt33-N (558bp) | PlyBt33-N/24kDa (amino acid residues 1–186) |
plyBt33-N-R/ SalI | GACGTCGACTGTAAACCAATCTAACGACT | Â | Â |
plyBt33-IC-F/BamHI | GAGGATCCCTTGGATACACTTCAAAAAT | plyBt33-IC (258bp) | PlyBt33-IC/11kDa (amino acid residues 187–272) |
plyBt33-R/ SalI | GACGTCGACTTCTTTTGTATAAAAGTATTTAA | Â | Â |