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Figure 3 | BMC Microbiology

Figure 3

From: Yersinia pestis insecticidal-like toxin complex (Tc) family proteins: characterization of expression, subcellular localization, and potential role in infection of the flea vector

Figure 3

Maximal synthesis of YitA and YipA during growth at low temperatures. A) KIM6+ (lanes 2, 5, 8, and 11), KIM6+ (pWKS130::yitR) (lanes 3, 6, 9, and 12) and KIM6+ (pCR-XL-TOPO::yitR) (lanes 4, 7, 10, and 13) grown overnight at 10°C, 22°C, 28°C or 37°C in BHI broth. YitA and YipA purified from E. coli (lane 15). B) KIM6+ (lanes 2, 5, 8, and 11), KIM6+ (pWKS130::yitR) (lanes 3, 6, 9, and 12) and KIM6+ (pCR-XL-TOPO::yitR) (lanes 4, 7, 10, and 13) grown overnight at 22°C or 37°C in either RPMI 1640 (RPMI) or whole sheep blood (Blood). YitA and YipA purified from E. coli (lanes 15 and 16). Panels show Western blots probed with anti-YitA, anti-YipA, or anti-Ail (sample loading control) antiserum.

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BMC Microbiology

ISSN: 1471-2180

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