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Figure 2 | BMC Microbiology

Figure 2

From: Yersinia pestis insecticidal-like toxin complex (Tc) family proteins: characterization of expression, subcellular localization, and potential role in infection of the flea vector

Figure 2

YitA and YipA are only detectable in Y. pestis isolated from fleas but over-production of YitR increases their synthesis in vitro . Lane 1, molecular weight ladder. Lane 2, Y. pestis KIM6+ isolated from infected fleas. Lane 3, KIM6+ΔyitA-yipB isolated from infected fleas. Lane 4, KIM6+ grown at 22°C in BHI. Lane 5, KIM6+ (pWKS130::yitR) grown at 22°C in BHI. Lane 6, KIM6+ (pCR-XL-TOPO::yitR) grown at 22°C in BHI. Lane 7, KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) grown at 22°C in BHI. Lanes 8–9, recombinant YitA and YipA purified from E. coli. Panels show Western blots probed with anti-YitA, anti-YipA, or anti-Ail (sample loading control) antiserum.

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BMC Microbiology

ISSN: 1471-2180

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