Table 1 Primers used in this study
From: Quorum-sensing regulates biofilm formation in Vibrio scophthalmi
Sequence (5’-3’) | Target gene | Reference | |
|---|---|---|---|
LuxR-A | GGACTAGTTACTAATTAGGGCAA | luxR null mutant | This study |
LuxR-B | ATAAATACACAACATGAGTCGGGTGCGGGG | “ | “ |
LuxR-C | ATGTTGTGTATTTATAAAGAAGAA | “ | “ |
LuxR-D | CTCGAGCTCGAGTCAGTGGGTCTA | “ | “ |
LuxR-G | CCGGAATTCCATTTGGCAAGGATT | Over expression luxR | “ |
LuxR-H | CGCGGATCCGGTGATGAGTTTCAC | “ | “ |
LuxR-1 | CCATTACACTCATAAGCGCGA | Sequencing luxR and | “ |
LuxR-2 | TCGAGATGGGTTGTGACGCTG | flanking regions | “ |
LuxRI-F2 | GCACCATTACACTCAT | Detection of luxR | “ |
LuxRI-R2 | TTTGATGAACATGTTTTG | “ | “ |
LuxRI-F4 | AAGTGTGGTTTGAGTGGA | Detection of luxR and | “ |
LuxRI-R4 | TAAGCAACAGCTGATGGA | flanking regions | “ |
LuxS-F6 | CGATCTTGCTCTACCGGCT | Sequencing luxS | “ |
LuxS-R7 | GAGTGCATCGCTGCAGTAC | flanking regions | “ |
LuxS-A | GGACTAGTCTGGCTTATCACGAAG | luxS null mutant | “ |
LuxS-B | CTCATTGAGCATTCGACAGTAAAGCTATC | “ | “ |
LuxS-C | GAAATGCTCAATGAGCTTCGCGTC | “ | “ |
LuxS-D | CTCGAGCTCGGACACTCGATCCACA | “ | “ |
LuxS-PMMBF | CCGGAATTCGCCAGCAGGAGAAGGACA | Over expression luxS | “ |
LuxS-PMMBR | CGCGGATCCCGCTATCGATTAATCGA | “ | “ |
LuxS-AI | GGATCCGCCAGCAGGAGAAGGACA | Cloning of luxS into pACYC184 | “ |
LuxS-BI | GTCGACCGCTATCGATTAATCGAC | “ |