AFM analysis of adhesion forces between C. albicans SC5314 and S. aureus NCTC8325-4GFP. Vertical scatter bars of adhesion forces between S. aureus NCTC8325-4GFP and different C. albicans strains and morphologies. (A) Different hyphal regions and yeast cells of C. albicans SC5314. (B) Different hyphal regions and yeast cells of C. albicans MB1. Each data point corresponds to a single force-distance curve recorded between a bacterium and a hypha. Median force values are indicated with a line. Statistically significant differences in adhesion forces (p < 0.05; Mann–Whitney test) of bacteria with the hyphal head region versus the middle or tip region are indicated by an asterisk.