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Table 3 Effect of various metal ions and chemical agent on the activity of the three enzymes

From: Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp. 1-7

Metal ion or chemical agent (5 mM)   Relative activity (%)  
  PdcDE PdcG PdcF
No addition 100 100 100
K + (KCl) 113.04 ± 10.80 95.79 ± 16.49 129.00 ± 27.32
Na + (NaCl) 113.42 ± 2.27 88.22 ± 17.76 123.91 ± 25.82
Ba 2+ (BaCl 2 ) 99.19 ± 6.29 123.34 ± 7.79 129.02 ± 6.46
Mg 2+ (MgCl 2 ) 95.41 ± 5.96 138.06 ± 8.46 129.79 ± 18.11
Zn 2+ (ZnCl 2 ) 87.44 ± 8.68 145.95 ± 5.13 21.44 ± 3.71
Cu 2+ (CuCl 2 ) 22.46 ± 6.83 110.18 ± 11.17 59.23 ± 12.57
Ni 2+ (NiCl 2 ) 111.05 ± 2.61 183.93 ± 30.68 35.25 ± 16.67
Co 2+ (CoCl 2 ) 104.15 ± 6.79 147.08 ± 17.51 79.14 ± 13.21
Mn 2+ (MnCl 2 ) 77.45 ± 2.93 186.12 ± 9.99 136.59 ± 3.65
Cd 2 + (CdSO 4 ) 63.24 ± 3.61 58.93 ± 3.88 39.52 ± 7.01
Fe 2+ (FeCl 2 ) 82.13 ± 13.46 39.47 ± 9.49 118.90 ± 21.53
Fe 3+ (FeCl 3 ) 78.33 ± 10.74 187.37 ± 15.37 134.89 ± 28.19
EDTA 62.44 ± 3.90 83.17 ± 8.32 112.93 ± 40.43
SDS 97.47 ± 1.65 81.58 ± 24.05 136.59 ± 3.66
  1. Each value represents the mean of at least three independent replicates.