Table 3 Effect of various metal ions and chemical agent on the activity of the three enzymes
Metal ion or chemical agent (5 mM) | Relative activity (%) | ||
|---|---|---|---|
PdcDE | PdcG | PdcF | |
No addition | 100 | 100 | 100 |
K + (KCl) | 113.04 ± 10.80 | 95.79 ± 16.49 | 129.00 ± 27.32 |
Na + (NaCl) | 113.42 ± 2.27 | 88.22 ± 17.76 | 123.91 ± 25.82 |
Ba 2+ (BaCl 2 ) | 99.19 ± 6.29 | 123.34 ± 7.79 | 129.02 ± 6.46 |
Mg 2+ (MgCl 2 ) | 95.41 ± 5.96 | 138.06 ± 8.46 | 129.79 ± 18.11 |
Zn 2+ (ZnCl 2 ) | 87.44 ± 8.68 | 145.95 ± 5.13 | 21.44 ± 3.71 |
Cu 2+ (CuCl 2 ) | 22.46 ± 6.83 | 110.18 ± 11.17 | 59.23 ± 12.57 |
Ni 2+ (NiCl 2 ) | 111.05 ± 2.61 | 183.93 ± 30.68 | 35.25 ± 16.67 |
Co 2+ (CoCl 2 ) | 104.15 ± 6.79 | 147.08 ± 17.51 | 79.14 ± 13.21 |
Mn 2+ (MnCl 2 ) | 77.45 ± 2.93 | 186.12 ± 9.99 | 136.59 ± 3.65 |
Cd 2 + (CdSO 4 ) | 63.24 ± 3.61 | 58.93 ± 3.88 | 39.52 ± 7.01 |
Fe 2+ (FeCl 2 ) | 82.13 ± 13.46 | 39.47 ± 9.49 | 118.90 ± 21.53 |
Fe 3+ (FeCl 3 ) | 78.33 ± 10.74 | 187.37 ± 15.37 | 134.89 ± 28.19 |
EDTA | 62.44 ± 3.90 | 83.17 ± 8.32 | 112.93 ± 40.43 |
SDS | 97.47 ± 1.65 | 81.58 ± 24.05 | 136.59 ± 3.66 |