Isolate used | Input bacteria (cfu/ml) | Adherent bacteria (cfu/ml) | Adherent bacteria as % of input | Invaded bacteria (cfu/ml) | Invaded bacteria as % of input | % Invaded/adherent (%I/IA) |
---|---|---|---|---|---|---|
Experiment 1 | ||||||
81-176 (+ve control) | (5.2 ± 0.8) × 107 | (3.3 ± 1.0) × 106 | 6.5 | (6.3 ± 2.2) × 105 | 1.22 | 18.8 |
00-2544 (+ prophage) | (3.7 ± 0.2) × 107 | (3.8 ± 1.2) × 105 | 1.0 | (2.7 ± 1.1) × 104 | 0.07 | 7.0 |
00-2538 (+ prophage) | (3.7 ± 0.9) × 107 | (8.7 ± 0.1) × 105 | 2.4 | (2.0 ± 0.8) × 105 | 0.54 | 23.0 |
00-2425 (+ prophage) | (4.1 ± 0.4) × 107 | (6.4 ± 0.8) × 105 | 1.6 | (2.0 ± 0.4) × 105 | 0.48 | 31.9 |
00-2426 (− prophage) | (4.1 ± 0.1) × 107 | (1.2 ± 0.4) × 105 | 0.3 | (1.0 ± 0.7) × 103 | 0.002 | 0.8 |
E. coli Top10 (invasion -ve) | (2.2 ± 0.1) × 107 | (5.2 ± 1.0) × 105 | 2.3 | (9.5 ± 0.2) × 103 | 0.042 | 1.8 |
Experiment 2 | ||||||
81-176 (+ve control) | (2.1 ± 0.5) × 107 | (3.5 ± 1.2) × 105 | 1.6 | (2.2 ± 0.3) × 105 | 1.04 | 64.3 |
00-2544 (+ prophage) | (1.8 ± 0.8) × 107 | (3.5 ± 2.4) × 105 | 1.9 | (6.5 ± 1.5) × 104 | 0.33 | 18.8 |
00-2538 (+ prophage) | (3.4 ± 0) × 107 | (3.9 ± 2.3) × 105 | 1.1 | (7.0 ± 0.9) × 104 | 0.20 | 17.9 |
00-2425 (+ prophage) | (3.3 ± 0.6) × 107 | (5.6 ± 1.5) × 105 | 1.7 | (8.4 ± 3.5) × 104 | 0.26 | 15.0 |
00-2426 (− prophage) | (3.4 ± 0.2) × 107 | (4.0 ± 2.1) × 104 | 0.1 | (8.7 ± 3.3) × 102 | 0.003 | 2.2 |
E. coli Top10 (invasion -ve) | (1.8 ± 0.2) × 107 | (1.7 ± 0.9) × 105 | 1.0 | (9.6 ± 1.6) × 103 | 0.055 | 5.7 |
Experiment 3 | ||||||
81-176 (+ve control) | (3.4 ± 0.1) × 107 | (1.8 ± 0.3) × 106 | 5.4 | (1.0 ± 0.6) × 105 | 0.30 | 5.6 |
00-2544 (+ prophage) | (2.3 ± 0.3) × 107 | (3.6 ± 1.3) × 105 | 1.6 | (4.1 ± 2.0) × 104 | 0.18 | 11.4 |
00-2538 (+ prophage) | (3.8 ± 0.4) × 107 | (6.3 ± 2.8) × 105 | 1.7 | (1.3 ± 0.3) × 105 | 0.33 | 20.2 |
00-2425 (+ prophage) | (4.3 ± 1.0) × 107 | (1.1 ± 0.2) × 106 | 2.5 | (2.5 ± 1.0) × 105 | 0.58 | 22.8 |
00-2426 (− prophage) | (3.8 ± 1.6) × 107 | (1.6 ± 0.3) × 105 | 0.4 | (5.5 ± 6.0) × 102 | 0.001 | 0.35 |
E. coli Top10 (invasion -ve) | (2.0 ± 0.01) × 107 | (2.1 ± 0.6) × 105 | 1.1 | (4.9 ± 2.2) × 103 | 0.024 | 2.3 |