Time course of PHB granule formation in R. eutropha H16 and HF39. Images of both strains are shown alternately after different times of PHB permissive growth as indicated. All preparations were performed as described in legend to Figure 1. Note, increased number of PHB granules in strain H16 compared to strain HF39 at longer growth times. Strain HF39 [(a) 0 min after transfer to fresh NB-gluconate medium; (d), 10 min after transfer; (f) 40 min and (i) 3 hours)]. Strain H16 [(b) 0 min after transfer to fresh NB-gluconate medium; (c) 10 min; (e) 30 min; (g) 1 hour and (h) 3 hours]. Size of bar as indicated.