Figure 6
Activity of isolimonic acid is dependent on QseBC . Inhibition of biofilm in (A) ΔqseBC mutant and ΔqseBC mutant complemented with qseBC (pVS178). (B) Biofilm formation in EHEC supplemented with qseBC, qseB and qseC. Asterisk denotes significant (p<0.05) difference from vector control. (C) Inhibition of biofilm by 100 μg/ml isolimonic acid in EHEC supplemented with qseBC, qseB and qseC. Asterisk denotes significant (p<0.05) difference from solvent control (DMSO). (D) Expression of qseB and qseC in presence of 100 μg/ml isolimonic acid. The fold changes in expression were calculated as isolimonic acid over DMSO. The experiments were conducted in triplicate and mean ± SD are presented.