Effect of a C-terminal deletion on HupF in R. leguminosarum hydrogenase processing. Immunodetection of hydrogenase large subunit HupL (top panels) and HypB (bottom panels) was carried out in crude extracts from vegetative cells induced for hydrogenase activity under different oxygen tensions (1% or 3%), and in bacteroid crude extracts. Strains: R. leguminosarum UPM1155 derivatives carrying plasmids pALPF5 (ΔhupF), pALPF5/pPM501 (hupFST), and pALPF5/pMP501C (hupF
). Proteins (60 μg for HupL and 10 μg for HypB) were resolved in 9% (HupL) or 12% (HypB) acrylamide SDS-PAGE gels.