Table 1 FungiQuant primer and probe sequences
From: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
FungiQuant (351 bp) | Tm (°C) | S. cerevisiaeregion | |
|---|---|---|---|
FungiQuant-F | 5′-GGRAAACTCACCAGGTCCAG-3′ | 60.5-62.5 | 1199-1218 |
FungiQuant-R | 5′-GSWCTATCCCCAKCACGA-3′ | 56.3-58.4 | 1269-1283 |
FungiQuant-Prb | (6FAM) 5′-TGGTGCATGGCCGTT-3′ (MGBNFQ) | 68.0 | 1532-1549 |