A-B. FungiQuant inter- and intra-run coefficient of variation (CoV). FungiQuant CoV is presented for copy number (solid line) and Ct-value (dashed line), demonstrating the range of CoV, which is lower for the 10 μl than the 5 μl reactions. For the 10 μl reactions, the FungiQuant intra-run copy number CoV is consistently below 15% until at 25 copies, and for the 5 μl reactions, the intra-run CoV is below 20% until at 50 copies. The FungiQuant Ct-value CoV is consistently below 10%, irrespective of reaction volumes.