Table 1 Strains and plasmids used in this study
Strain and plasmid | Genotype or description | Source or reference |
|---|---|---|
Strains | Â | Â |
Streptomyces strains (Y27, Y32, Y33, Y34, Y41, Y42 and G2-1) | Isolated from Gingko harboring pWTY27 | This work |
Streptomyces strains(W15, W24, W37 and W41) | Isolated from Artemisia annua L harboring pWTY27 | This work |
Streptomyces strains (Z20, Z54 and Z70) | Isolated from Taxus harboring pWTY27 | This work |
S. lividans ZX7 | pro-2 str-6 rec-46 dnd SLP2- SLP3- | 34 |
S. coelicolor A3(2) | SCP1 SCP2 | 35 |
Escherichia coli DH5α | F- deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Invitrogen |
E. coli BL21 (DE3) | hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen |
E. coli ET12567 (pUZ8002) | dam dcm hsdM cm kan | 35 |
Plasmids | Â | Â |
pSP72 | amp colEI ori | Invitrogen |
pIJ702 | tsr melC pIJ101 ori | 39 |
pYQ1 | A 14-kb SacI-fragment cloned in pSP72 | This work |
pQC156 | A 2.6-kb BclI-fragment of melC/tsr cloned in pSP72 (BglII) | 26 |
pZR131 | Two 381-bp telomeres tsr melC amp colEI ori | 8 |
pWT177 | A 3.8-kb fragment (100-3941 bp) cloned in pZR131 (EcoRI) | This work |
pSET152 | amp apr oriT int(phiC31) | 36 |
pWT181 | pSET152 derivative, amp tsr melC cos oriT int(phiC31) | This work |
pET28b | kan | Novagen |
pWT111 | A 1.6-kb fragment (574-2253 bp of pWTY27) cloned in pET28b (EcoRI+HindIII) | This work |
pWT371 | A 1.7-kb fragment (8124-9836 bp of pWTY27) cloned in pET28b (NheI+HindIII) | This work |
pFX144 | A 1.3-kb fragment (37-1328 bp of pIJ773 containing oriT/apr) cloned in pSP72 (XbaI) | This work |
pWT26 | A 1.3-kb fragment (13-1369 bp of pFX144 containing oriT/apr) cloned in pYQ1(EcoRV) | This work |
pWT24 | A 5.4-kb fragment (13942-14288/1-5114 bp of pWTY27) cloned in pFX144 (SspI + SacI) | This work |
pWT147 | A 3.8-kb fragment (100-3941 bp) cloned in pFX144 (XbaI) | This work |
pWT219 | A 3.2-kb fragment (321-3506 bp) cloned in pFX144 (XbaI) | This work |
pWT217 | A 1.9-kb fragment (321-2267 bp) cloned in pFX144 (XbaI) | This work |
pWT222 | A 2.9-kb fragment (621-3506 bp) cloned in pFX144 (XbaI) | This work |
pWT223 | A 0.3-kb fragment (321-620 bp) containing iteron cloned in pWT222 (BamHI) | This work |
pWT241 | A 0.15-kb fragment (382-530 bp) containing iteron cloned in pWT224 (BamHI) | This work |
pWT34 | A 95-bp fragment (1073-1167 bp) deleted from pWT24 | This work |
pWT33 | A 259-bp fragment (2433-2691 bp) deleted from pWT24 | This work |
pWT203 | A 6-kb fragment containing the rep/rlrA/rorA of pSLA2 cloned in pFX144 (PvuII) | This work |
pWT208 | A 3.2-kb fragment (6757-9977 bp) cloned in pWT203 (SspI) | This work |
pWT207 | A 1.5-kb fragment (6757-8270 bp) cloned in pWT203 (SspI) | This work |
pWT210 | A 2.2-kb fragment (7734-9977 bp) cloned in pWT203 (SspI) | This work |
pWT225 | A 2.2-kb fragment (7734-9893 bp) cloned in | This work |
pWT224 | pWT203 (SspI) | This work |
A 2.1-kb fragment (7734-9818 bp) cloned in pWT203 (SspI) | Â | |
pWT242 | A 175-bp fragment (9803-9977 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT262 | A 46-bp fragment (9803-9848 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT231 | A 87-bp fragment (9803-9889 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT229 | A 100-bp fragment (9803-9902 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT239 | A 128-bp fragment (9803-9930 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT238 | A 150-bp fragment (9803-9952 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT251 | A 134-bp fragment (9844-9977 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT259 | A 165-bp fragment (9813-9977 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |
pWT265 | A 159-bp fragment (9819-9977 bp) cloned in | This work |
| Â | pWT224 (SspI) | Â |