Figure 5

PCR-based analysis of cyp61^-mutants. Each gel shows PCR reactions performed with different sets of primers and genomic DNA from strains UCD 67–385 (lane 1), 385-CYP61/cyp61^hph (lane 2), 385-cyp61^hph/cyp61^zeo (lane 3), CBS 6938 (lane 4), CBS-cyp61^hph(lane 5), AVHN2 (lane 6), Av2-cyp61^zeo (lane 7), and a negative control without DNA (lane 8). The diagram below each gel represents the amplification target (cyp61^- mutant or wild-type allele) and the size of the expected amplicon. The colors represent the resitance cassettes HygR in green and ZeoR in violet, the CYP61 gene in red and the CYP61 flanking DNA in dark grey. The EcoRV recognition site, where the respective antibiotic resistance marker was inserted to disrupt the CYP61 gene, is also shown. Molecular weight standard were: lambda DNA/Hind III (23.1, 9.4, 6.6, 4.4, 2.3, 2.0 and 0.6 kbp) at the left, and 1 kb DNA ladder (10.0, 8.0, 6.0, 5.0, 4.0, 3.5, 3.0, 2.5, 2.0, 1.5, 1.0, 0.75 and 0.5 kbp) at the right, of each gel.