Table 1 Oligonucleotide primers used in this study
Application | Name | Oligonucleotide sequence (5’ to 3’) |
|---|---|---|
Strain construction | PKD13sspAUS2 | ACTATCATCCAATTTTCTGCCCAAATGTCGGGTATTGCTCAGGAGGTTTCTTTCATGATGTCCGGGGATCCGTCGACCTGC |
| Â | PKD13sspADS | AGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTGTAGGCTGGAGCTGCTTCGA |
| Â | sspABUS | GATTGGATCCGGCTTCGTTACTCGTGACGCT |
| Â | sspABDS | GATTGAAGCTTACTTCACAACGCGTAATGC |
|  | hnsΔ92-1 | GAGCTGCTGAATAGCCTTGCCGCCGTTAAATCTGGCACCAAAGCTAAACGTGCTTGTGTAGGCTGGAGCTGCTTCG |
|  | hnsΔ92-2 | GGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTTATGAATATCCTCCTTAGTTC |
| Â | hnsUS2 | CCATGGAATTCACCATGAGCGAAGCACTTAAAATTCTGAACA |
| Â | hnsDS2 | CCAGGTCTGCAGTTATTGCTTGATCAGGAAATCG |
Plasmid construction | lerUS2 | GATTGGATCCGCTGCGACTGCGTTCGCTTGCT |
| Â | lerDS2 | GATTGAAGCTTCCAGCTCAGTTATCGTTAT |
Primer extension | lerPE | GCTTCCTGCTGTAGA |
| Â | LEE2PE | GCTGCTTCCATTGATCTTTCTCC |
| Â | LEE3PE | CAATTTCAACACGGTTATC |
| Â | LEE4PE | CAGATGCGGGGTTTTGATTAAATTC |
| Â | LEE5PE | CATTGGGATTATGACCAAGA |
| Â | mapPE | GGACTAAACATGCTATAAACC |
| Â | grlRPE | CCTTCCCCACAGGAGTCTTC |
| Â | stcEPE | GGCAAGGATCGTACATGA |
| Â | ompAPE | ACGAGATAACACGGTTAAATCC |