|
Strain construction
|
PKD13sspAUS2
|
ACTATCATCCAATTTTCTGCCCAAATGTCGGGTATTGCTCAGGAGGTTTCTTTCATGATGTCCGGGGATCCGTCGACCTGC
|
| |
PKD13sspADS
|
AGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTGTAGGCTGGAGCTGCTTCGA
|
| |
sspABUS
|
GATTGGATCCGGCTTCGTTACTCGTGACGCT
|
| |
sspABDS
|
GATTGAAGCTTACTTCACAACGCGTAATGC
|
| |
hnsΔ92-1
|
GAGCTGCTGAATAGCCTTGCCGCCGTTAAATCTGGCACCAAAGCTAAACGTGCTTGTGTAGGCTGGAGCTGCTTCG
|
| |
hnsΔ92-2
|
GGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTTATGAATATCCTCCTTAGTTC
|
| |
hnsUS2
|
CCATGGAATTCACCATGAGCGAAGCACTTAAAATTCTGAACA
|
| |
hnsDS2
|
CCAGGTCTGCAGTTATTGCTTGATCAGGAAATCG
|
|
Plasmid construction
|
lerUS2
|
GATTGGATCCGCTGCGACTGCGTTCGCTTGCT
|
| |
lerDS2
|
GATTGAAGCTTCCAGCTCAGTTATCGTTAT
|
|
Primer extension
|
lerPE
|
GCTTCCTGCTGTAGA
|
| |
LEE2PE
|
GCTGCTTCCATTGATCTTTCTCC
|
| |
LEE3PE
|
CAATTTCAACACGGTTATC
|
| |
LEE4PE
|
CAGATGCGGGGTTTTGATTAAATTC
|
| |
LEE5PE
|
CATTGGGATTATGACCAAGA
|
| |
mapPE
|
GGACTAAACATGCTATAAACC
|
| |
grlRPE
|
CCTTCCCCACAGGAGTCTTC
|
| |
stcEPE
|
GGCAAGGATCGTACATGA
|
| |
ompAPE
|
ACGAGATAACACGGTTAAATCC
|
