The gluQ-rs mutant is sensitive to growth in high osmolarity. Biolog phenotypic MicroArrays were used to characterize the growth and metabolism of the gluQ-rs mutant and its wild type parent, 2457T. Wild type (black line) transformed with the empty plasmid, 2457T ΔgluQ-rs::kan (red line) transformed with the empty plasmid pCM and S. flexneri ΔgluQ-rs::kan (blue line) transformed with the complementary plasmid pTRCGQ were incubated for 24 h with medium in the corresponding plate for osmolytes (PM9). A) sodium chloride 1% B) sodium benzoate 20 mM pH 5.2. C) sodium nitrate 100 mM. Metabolism was monitored by measuring reduction of the tetrazolium dye in the medium at 15 min intervals and is shown as units.