Table 1 Oligonucleotides used in this study

essB-attB1 -F

GGGGACAAGTTTGTACAAAAAAGCAGGCTCATCTTAATGGTGATTTTAACTATG

Cloning of the essB deletion mutant in pKOR1 for allelic replacement

essB -15codons-R

AAAGATCTTAACATATCTTGCATTTCATTTTTAG

Same as above

essB -15codons-F

AAAGATCTCAAAAAGATAAAGAAAAACGCCAAG

Same as above

essB-attB2 -R

GGGGACCACTTTGTACAAGAAAGCTGGGTCAGCAACCGTTGGATAATGGTAATTC

Same as above

essB-Xho I-F

AAACTCGAGATGGTTAAAAATCATAACCCTAAAAATGAA

Gene expression in E. coli or S. aureus using pET15b or pWWW412, respectively

essB-BamH I-R

AAAGGATCCCTATTTTTTTCTTTCAGCTTCTTGGCGTTT

Same as above

essB(1–223)-BamH I-R

AAAGGATCCCTACCCTACTTTGCGTACATATGCATAA

Same as above

essB(253–444)-Nde I-F

AAACATATGAAGCATAATGAGCGCATTGAAAAAG

Same as above

essB(1–252)- BamH I-R

AAAGGATCCCTATACTGAAAAATATAAAAAGGCTAAAAAT

Same as above

essB(220–444)-Nde I-F

AAACATATGCGCAAAGTAGGGCATACCGTTTTCAAA

Same as above

essB (Δ224-252)- EcoR I-F

AAAGAATTCATGAAGCATAATGAGCGCATTGAAAAAG

Same as above

essB (Δ224-252)- EcoR I-R

AAAGAATTCTCATGGGTTCACCCTATCAAGCCCTTGCTT

Same as above

essB-BamH I-F

AAAGGATCCATGGTTAAAAATCATAACCCTAAAAAT

Production of GST hybrids using pGEX-2TK

essB-EcoR I-R

AAAGAATTCCTATTTTTTTCTTTCAGCTTCTTGGCGT

Same as above

essB(1–223)-EcoR I-R

AAAGAATTCCTACCCTACTTTGCGTACATATGCATAA

Same as above

essB(253–444)-BamH I-F

AAAGGAATCATGAAGCATAATGAGCGCATTGAAAAAG

Same as above