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Table 2 Physiological properties of C. defragrans strains growing with different monoterpenes

From: Physiology of deletion mutants in the anaerobic β-myrcene degradation pathway in Castellaniella defragrans

  α-Phellandrene Limonene β-Myrcene
65Phen ΔgeoA ΔgeoA comp 65Phen ΔgeoA ΔgeoA comp 65Phen ΔgeoA ΔgeoA comp
MaxOD660 0.321 0.217 0.342 0.318 0.174 0.347 0.155 0.066 0.149
Generation time [h] 9.8 34.9 13.5 25.4 50.8 44.9 46.9 57.1 45.8
NO3- consumed [mM] 10 10 10 10 10 10 7.3 5.8 8.1
NO2- formed [mM] 0 0 0 0 0 0.01 0.22 0 0.009
Biomass formed [g/L] 0.34 0.23 0.32 0.35 0.22 0.35 0.14 0.08 0.17
  1. C. defragrans strains 65Phen (wild type), Δgeo A and Δgeo A comp were grown under standard conditions at 28°C for 280 h (α-phellandrene, limonene) or for 304 h (β-myrcene) with 4 mM monoterpene (in HMN) and 10 mM nitrate. As negative control served a culture without inoculum.