Physiology of deletion mutants in the anaerobic β-myrcene degradation pathway in Castellaniella defragrans

BMC Microbiology

Table 1 Geranic acid pools in cultivation media

*C. defragrans strains*	Geranic acid concentration [μM]
*C. defragrans strains*	α-Phellandrene	β-Myrcene
65Phen (wild type)	0.24 ± 0.01	8.85 ± 0.6
Δldi	n.d.	n.d.
Δldi comp	0.33 ± 0.24	6.61 ± 0.19
ΔgeoA	n.d.	4.96 ± 1.58
ΔgeoA comp	0.89 ± 0.25	11.79 ± 0.31

C. defragrans cultures were grown in 150 mL with 6 mM α-phellandrene or β-myrcene and 10 mM nitrate at 30°C and 130 rpm. Inoculum size was 1% (v/v). Duplicate determination. Detection limit for geranic acid was 6.4 nM. n.d. = not detectable.

ISSN: 1471-2180